Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure

نویسندگان

  • Svenja Staudt
  • Christina A Müller
  • Jan Marienhagen
  • Christian Böing
  • Stefan Buchholz
  • Ulrich Schwaneberg
  • Harald Gröger
چکیده

The hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the "liquid gas" n-butane with in situ cofactor regeneration, which does not require high-pressure conditions, was developed. The resulting 2-butanol was obtained as the only regioisomer, at a product concentration of 0.16 g/L.

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عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2012